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Literature summary extracted from

  • Zuber, H.
    Carboxypeptidase C (1976), Methods Enzymol., 45, 561-568.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.16.5 orange peel enzyme is inactivated upon lyophilization Citrus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.5 diisopropyl fluorophosphate peel enzyme inhibited, leaf enzyme not Citrus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.5 0.5
-
 benzyloxycarbonyl-Leu-Phe orange peel Citrus sp.
3.4.16.5 4
-
 benzyloxycarbonyl-Arg-Pro orange peel Citrus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.16.5 Zinc possibly contains zinc in the active site, orange peel Citrus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.16.5 86000
-
 enzyme from peel, gel filtration Citrus sp.
3.4.16.5 175000
-
 enzyme from leaf, gel filtration Citrus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.5 Citrus sp.
-
-
-
3.4.16.5 Citrus sp.
-
 orange
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.5
-
 Citrus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.16.5 leaf
-
 Citrus sp.
-
3.4.16.5 peel flavedo Citrus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.16.5 additional information
-
-
 Citrus sp.

Storage Stability

EC Number Storage Stability Organism
3.4.16.5 -20°C, pH 4.7-5.5, stable for several months Citrus sp.
3.4.16.5 4°C, pH 4.7-5.5, stable for a few days Citrus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.5 benzyloxycarbonyl-Arg-Pro + H2O
-
 Citrus sp. benzyloxycarbonyl-Arg + Pro
-
 ?
3.4.16.5 benzyloxycarbonyl-Leu-Phe + H2O
-
 Citrus sp. benzyloxycarbonyl-Leu + Phe
-
 ?
3.4.16.5 additional information splits off most readily aromatic and aliphatic amino acid residues at the carboxyl end Citrus sp. ?
-
 ?
3.4.16.5 additional information glycine is split off very slowly Citrus sp. ?
-
 ?
3.4.16.5 additional information neutral, basic and acid amino acids including proline are all hydrolyzed to approximately the same extent Citrus sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.5 5.3 5.7 benzyloxycarbonyl-Leu-Phe, benzyloxycarbonyl-Arg-Pro, orange peel Citrus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.16.5 4 6 orange leaf enzyme is unstable below pH 4 and above pH 6 Citrus sp.
3.4.16.5 4 6 stable, irreversible inactivation above pH 6, orange peel Citrus sp.
3.4.16.5 5
-
 most stable at, orange leaf enzyme Citrus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.16.5 Citrus sp. orange leaf
-
 4.5